Colina tested positive on Friday and the department has since then appointed Deputy Chief Ronald Papier as the acting police chief, CNN affiliate WSVN reported.
More than 706,000 people have tested positive for the virus in the US. At least 37,079 have died.
“My spirits are high and I have every reason to believe that I will have a full recovery,” Colina said in a statement shared with the news station. He added that his symptoms were mild.
“I am following our internal policies and entering self-isolation until follow-up tests determine that I am no longer at risk for spreading the virus to coworkers,” he said.
“For your safety and that of your fellow employees, please continue to follow our departmental directives regarding personal protective measures and social distancing,” he added.
In March, Colina had been instructed to self-isolate, after the city’s mayor, Francis Suarez, tested positive, CNN affiliate WKMG reported.
By March 14, Colina announced on Twitter he had tested negative for the virus and would be returning to his duties.